Optimized DLLME Method Coupled to HPLC for Simultaneous Analyses of Benzoates, Sorbates and Methyl p-hydroxyl Benzoates in Fruits Products

Abstract

We used an accurate and green technique "Dispersive Liquid Liquid Micro Extraction" (DLLME) followed by HPLC-UV analysis for the analysis of benzoates (BA), sorbates (SA), and p-hydroxyl methyl benzoates (MB) in fruits products. We optimized the   extraction parameters by the box Behnken design. We validated the method by using the β accuracy profile including the systematic (trueness) and random (intermediate precision) errors. The extraction efficiency varied from 82.98 to 100.8 %. This method was linear with R²s values higher than 0.9752 for all the compounds. The repeatability and intermediate precision were less than 3.18 and 12.02 respectively; whereas, the detection limits were respectively 0.304, 0.306 and 0.153 mg/ ml for BA, SA and MB. This method is therefore reliable to the simultaneous quantification of these preservatives in fruit juices, nectars and jams. The levels of these additives in the samples exceeded 833.97 and 290.67 ppm for BA and SA, respectively; while, MB was not detected.